Hening Lin

Frank and Robert Laughlin Professor of Physical Chemistry

Publications

Jing H, and Lin H. “Sirtuins in Epigenetic Regulation”, Chem. Rev., 115, 2350-2375, 2015
Liu X, Sadhukhan S, Sun S, Wagner GR, Hirschey MD, Qi L, Lin H, Locasale JW, “High resolution metabolomics with acyl-CoA profiling reveals widespread remodeling in response to diet.” Mol. Cell. Proteomics 2015, mcp.M114.044859
Teng YB, Jing H, Aramsangtienchai P, He B, Khan S, Hu J, Lin H, Hao Q, “Efficient demyristoylase activity of SIRT2 revealed by kinetic and structural studies.” Sci. Rep. 5, 8529, 2015. doi: 10.1038/srep08529.
Otwell AE, Sherwood RW, Zhang S, Nelson OD, Li Z, Lin H, Callister SJ, Richardson RE, “Identification of proteins capable of metal reduction from the proteome of the Gram-positive bacterium Desulfotomaculum reducens MI-1 using an NADH-based activity assay.” Environ. Microbiol. 2014, doi: 10.1111/1462-2920.12673. [Epub ahead of print]
He B, Hu J, Zhang X, and Lin H. “Thiomyristoyl peptides as cell-permeable Sirt6 inhibitors”, Org. Biomol. Chem., 12, 7498-7502, 2014
Lin Z, Su X, Chen W, Ci B, Zhang S, Lin H. Dph7 catalyzes a previously unknown demethylation step in diphthamide biosynthesis. J Am Chem Soc. 2014
Shrimp JH, Hu J, Dong M, Wang BS, Macdonald RJ, Jiang H, Hao Q, Yen A, Lin H. Revealing CD38 Cellular Localization Using a Cell Permeable, Mechanism-Based Fluorescent Small Molecule Probe. J Am Chem Soc. 2014
Dong M, Su X, Dzikovski B, Dando EE, Zhu X, Du J, Freed JH, Lin H. Dph3 is an electron donor for dph1-dph2 in the first step of eukaryotic diphthamide biosynthesis. J Am Chem Soc. 136, 1754-7, 2014
G. Colak, Z. Xie, A. Zhu, L. Dai, Z. Lu, Y. Zhang, X. Wan, Y. Chen, Y.H. Cha, H. Lin, Y. Zhao, and M. Tan, “Identification of Lysine Succinylation Substrates and the Succinylation Regulatory Enzyme CobB in Escherichia coli”. Mol. Cell. Proteomics, 12, 3509-3520, 2013
Yu J, Sadhukhan S, Noriega LG, Moullan N, He B, Weiss RS, Lin H, Schoonjans K, Auwerx J. “Metabolic Characterization of a Sirt5 deficient mouse model”, Sci. Rep. 3, 2806, 2013.
Su X, Lin Z, Lin H. The biosynthesis and biological function of diphthamide. Crit Rev Biochem Mol Biol. 2013
Hu J, He B, Bhargava S, Lin H. A fluorogenic assay for screening Sirt6 modulators. Org Biomol Chem. 11, 5213-6, 2013
Jiang H, Khan S, Wang Y, Charron G, He B, Sebastian C, Du J, Kim R, Ge E, Mostoslavsky R, Hang HC, Hao Q, Lin H. SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. Nature. 496, 110-3. 2013
Jiang H, Lin H. Labeling Substrate Proteins of Poly(ADP-ribose) Polymerases with Clickable NAD Analog. Curr Protoc Chem Biol. 4, 19-34, 2012
Su X, Lin Z, Chen W, Jiang H, Zhang S, Lin H. Chemogenomic approach identified yeast YLR143W as diphthamide synthetase. Proc Natl Acad Sci U S A. 109, 19983-7, 2012
Jiang H, Sherwood R, Zhang S, Zhu X, Liu Q, Graeff R, Kriksunov IA, Lee HC, Hao Q, Lin H. Identification of ADP-ribosylation sites of CD38 mutants by precursor ion scanning mass spectrometry. Anal Biochem. 433, 218-26. 2012
Zhou Y, Zhang H, He B, Du J, Lin H, Cerione RA, Hao Q. The bicyclic intermediate structure provides insights into the desuccinylation mechanism of human sirtuin 5 (SIRT5). J Biol Chem. 287, 28307-14, 2012
Lin H, Su X, He B. Protein lysine acylation and cysteine succination by intermediates of energy metabolism, ACS Chem Biol. 7, 947-60, 2012
He B, Du J, Lin H. Thiosuccinyl peptides as Sirt5-specific inhibitors, J Am Chem Soc, 134, 1922-5, 2012
Su X, Chen W, Lee W, Jiang H, Zhang S, Lin H. YBR246W is required for the third step of diphthamide biosynthesis, J Am Chem Soc, 134(2), 773-6, 2012
Zhu AY, Zhou Y, Khan S, Deitsch KW, Hao Q, Lin H. Plasmodium falciparum Sir2A preferentially hydrolyzes medium and long chain fatty acyl lysine, ACS Chem Biol. 7, 155-9, 2012
J. Du, Y. Zhou, X. Su, J. Yu, S. Khan, H. Jiang, J. Kim, J. Woo, J. Kim, B. Choi, B. He, W. Chen, S. Zhang, R. Cerione, J. Auwerx, Q. Hao, and H.Lin. Sirt5 is a NAD-Dependent Protein Lysine Demalonylase and Desuccinylase, Science. Vol. 334, No 6507, 806-809, 2011
H. Lin, “S-Adenosylmethionine-dependent alkylation reactions: When are radical reactions used?” Bioorg. Chem. 39, 161-170 (2011).
X. Zhu, B. Dzikovski, X. Su, A.T. Torelli, Y. Zhang, S.E. Ealick, J.H. Freed, and H. Lin. Mechanistic understanding of Pyrococcus horikoshii Dph2, a [4Fe-4S] enzyme required for diphthamide biosynthesis, Mol. BioSystems. 7, 74-81, 2011
J. Congleton, H. Jiang, F. Malavasi, H. Lin, A. Yen, “ATRA-induced HL-60 myeloid leukemia cell differentiation depends on the CD38 cytosolic tail needed for membrane localization, but CD38 enzymatic activity is unnecessary” Exp. Cell Res. 317, 910-919 (2011).
X. Zhu, J. Kim, X. Su, and H. Lin. Reconstitution of diphthine synthase activity in vitro, Biochemistry. 49, 9649-9657, 2010
Hong Jiang, Jun Hyun Kim, Kristine M. Frizzell, W. Lee Kraus and Hening Lin;Clickable NAD Analogues for Labeling Substrate Proteins of Poly(ADP-ribose) Polymerases, JACS. (2010)
Yang Zhang, Xuling Zhu, Andrew T. Torelli, Michael Lee, Boris Dzikovski, Rachel M. Koralewski, Eileen Wang, Jack Freed, Carsten Krebs, Steven E. Ealick & Hening Lin;Diphthamide biosynthesis requires an organic radical generated by an iron–sulphur enzyme, Nature. 465,891-896 (2010)
Q. Liu, R. Graeff, I. A. Kriksunov, H. Jiang, B. Zhang, B. V. L. Potter, N. Oppenheimer, H. Lin, H. C. Lee, Q. Hao, “Structural Basis for Enzymatic Evolution from a Dedicated ADP-ribosyl Cyclase to a Multi- functional NAD Hydrolase”, J. Biol. Chem., 284, 27637-27645, (2009).
H. Jiang, J. Congleton, Q. Liu, P. Merchant, F. Malavasi, H.C. Lee, Q. Hao, A. Yen, H. Lin, “Mechanism-Based Small Molecule Probes for Labeling CD38 on Live Cells”, J. Am. Chem. Soc., 131, 1658-1659 (2009) Highlighted in Nat. Chem. Biol. 5, 149 (2009) Link
J. Du, H. Jiang, H. Lin, “Investigating the ADP-ribosyltransferase activity of sirtuins with NAD analogs and 32P-NAD”, Biochemistry 48, 2878–2890 (2009)
Q. Liu, I.A. Kriksunov, H. Jiang, R. Graeff, H. Lin, H.C. Lee, Q. Hao,“Covalent and non-covalent intermediates of an NAD utilizing enzyme – human CD38”, Chem. Biol. 15,1068-78 (2008)
H. Lin, J. Du, H. Jiang, “Posttranslational modifications to regulate protein function”, Wiley Encyclopedia of Chemical Biology, 2008. John Wiley & Sons, Inc. DOI: 10.1002/9780470048672. wecb467
H. Lin, “Nicotinamide adenine dinucleotide: beyond a redox coenzyme”, Org. Biomol. Chem., 5, 2541-2554 (2007)

Jeremy M. Baskin

Associate Professor

Publications

Please see a complete publication list on the Baskin Lab website.

Selected publications from the Baskin Lab:

Bumpus TW, Huang S, Tei R, and Baskin JM. “Click chemistry–enabled CRISPR screening reveals GSK3 as a regulator of PLD signaling.” Proc Natl Acad Sci USA (2021) 118, 48, e2025265118.
Tei R*, Morstein J*, Shemet A, Trauner D^†, and Baskin JM^†. “Optical control of phosphatidic acid signaling.” ACS Cent Sci (2021) 7, 7, 1205–1215. *Equal contribution; ^†Co-corresponding authors.
Liang D, Cheloha R, Watanabe T, Gardella TJ, and Baskin JM. “Activity-based, bioorthogonal imaging of phospholipase D reveals spatiotemporal dynamics of GPCR-Gq signaling.” Cell Chem Biol (2021) 28, 1–7.
Shami Shah A, Cao X, White AC, Baskin JM. “PLEKHA4 Promotes Wnt/β-catenin Signaling-Mediated G1/S Transition and Proliferation in Melanoma.” Cancer Res (2021) 81, 2029–43.
Tei R and Baskin JM. “Spatiotemporal Control of Phosphatidic Acid Signaling with Optogenetic, Engineered Phospholipase Ds.” J Cell Biol (2020) 219, 3, e201907013.
Liang D, Wu K*, Tei R*, Bumpus TW, Ye J, and Baskin JM. “A real-time, click chemistry imaging approach reveals stimulus-specific subcellular locations of phospholipase D activity.” Proc Natl Acad Sci USA (2019) 116, 31, 15453–62. *Equal contribution.
Shami Shah A, Batrouni AG, Kim D, Punyala A, Cao W, Han ML, Smolka MB, and Baskin JM. “PLEKHA4/kramer attenuates Dishevelled ubiquitination to modulate Wnt and planar cell polarity signaling.” Cell Rep (2019) 27, 2157–70.
Bumpus TW and Baskin JM. “Clickable Substrate Mimics Enable Imaging of Phospholipase D Activity.” ACS Cent Sci (2017) 3, 10, 1070-1077.
Bumpus TW and Baskin JM. “A Chemoenzymatic Strategy for Imaging Cellular Phosphatidic Acid Synthesis.” Angew Chem Int Ed (2016) 55, 13155-13158.

G60 ST Olin
jeremy.baskin@cornell.edu
607/255-2887

Education

Postdoc, Yale University
PhD, University of California, Berkeley
BS, Massachusetts Institute of Technology

Departments and programs

Chemistry and Chemical Biology

Keywords

Biochemistry, Cell Biology, Chemical Biology, Imaging Probes, Lipids and Membranes

Mikail Abbasov

Assistant Professor

Education

Departments and programs

Links

Keywords

Lara A. Estroff

Professor

Education

Departments and programs

Links

Pamela Chang

Associate Professor

Publications

Education

Departments and programs

Links

Dotsevi Y. Sogah

Professor Emeritus

Publications

Education

Departments and programs

Keywords

Frank C. Schroeder

Professor

Publications

Education

Departments and programs

Links

Keywords

Hening Lin

Frank and Robert Laughlin Professor of Physical Chemistry

Publications

Education

Departments and programs

Links

Jeremy M. Baskin

Associate Professor

Publications

Education

Departments and programs

Links

Keywords